By Kathleen Cross M'Closkey
Read or Download [Dissertation] Myths, Markets, and Metaphors: Navajo Weaving as Commodity and Communicative Form PDF
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Extra resources for [Dissertation] Myths, Markets, and Metaphors: Navajo Weaving as Commodity and Communicative Form
The chemical structure of MDC is also shown below the fluorescence gel image. Reproduced with permission from ref. 3 © 2009 John Wiley & Sons, Inc. Place the clamp assembly in the casting cradle, with the screws facing outward. In this position, the gel will be visible through the rectangular glass plate. Ensure that there is a good seal between the bottom of the gel sandwich stack and the rubber gasket in the casting cradle. 2 ml), TEMED (10 ml), and APS (20 ml). Pour the separating gel into the sandwich stack.
6. 5). 1, items 2–10. 3-Prop-2-ynoxyprop-1-yne (dipropargyl ether). Copper sulfate pentahydrate (CuSO4⋅5H2O). Sodium ascorbate. Tert-Butanol (t-BuOH). Tetrahydrofuran (THF). Milli-Q water (Millipore). (see 32 Dolai et al. Dialysis tubing (10 kDa MWCO). Sephadex™ LH-20 gel filtration chromatography medium (GE Healthcare). 7. Bovine serum albumin (BSA). 2,5-Dioxopyrrolidin-1-yl-5-azidopentanoate (NHS-azide). This reagent can be synthesized according to the procedure previously reported in ref.
9. A very important point to note is the temperature shift prior to induction (from 37°C to 24°C) to prevent the formation of inclusion bodies. This step reduces the transcription and translation velocity, delivering the pro-enzyme in limited amounts and enabling the protein to fold properly. Alternatively, the autoinduction medium (9) can be used at an incubation temperature of 28°C. Inactive pro-TG enzyme can be purified using metal affinity chromatography and is activated prior to use by removal of the pro-sequence using a suitable protease, such as dispase (8).